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Table 1 Cytochemical stains used to identify the lineage of myeloid cells [6, 46]

From: A review of current knowledge of myeloproliferative disorders in the horse

Cytochemical reaction stain

Cellular element stained

Blasts/cell type identified

Myeloperoxidase (MPO)

Neutrophil primary granules

Myeloblasts strong + /monoblasts faint + 

Neutrophil; Monocyte

Sudan Blanck B (SBB)

Phospholipids

Myeloblasts strong + /monoblasts faint + 

Neutrophil; Monocyte

Naphtol AS-D chloroacetate esterase (CAE); specific

Esterese enzymes

Myeloblasts strong + 

Granulocytes

Leukocyte alkaline phosphatase (ALP)

Neutrophil secondary granules

Metamyelocytes, band and segmented neutrophil

Monocyte

Alpha naphthyl acetate esterase (ANAE); non-specific

Alpha naphthyl butyrate esterase (ANBE); non-specific

Esterase enzymes

Monoblasts strong + 

Monocyte strong + 

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Acta Veterinaria Scandinavica

ISSN: 1751-0147